recombinant DNA

Construction of the Recombinant HBV DNA Vaccines by Insertion ISS and Immunogenicity of It

插入ISS的HBV dna疫苗的构建及其免疫原性

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Using recombinant glucagon expression system, the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis. Glucagon gene mutation was testified by DNA sequencing.

在获得重组胰高血糖素基因工程菌基础上，利用定点突变技术改造其第21位氨基酸天冬氨酸为丙氨酸，并经DNA测序证明胰高血糖素基因发生了点突变。

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The relationship between template activity and structural variation of recombinant plasmid DNA was studied in a cell-free system for in vitro transcription which was developed with maize RNA polymerase B and recombinant plasmid DNA.

本文以重组质粒pZmS51DNA为转录模板，在无细胞离体转录系统中研究了质粒DNA的构型变化与其模板活性的关系。

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42K envelope protein gene of Contagious Ecthyma virus were amplified by specification primers and recombinant to different expression vector. The 9 strains stable recombinant were screened by dot blotting and southern blotting with DNA probe labeled DIG.

用特异性引物扩增羊传染性脓疱病毒42K囊膜蛋白基因，与不同的表达载体重组后用DIG标记的特异性DNA探针进行Dot-blotting和southern blotting杂交检测，挑选出能稳定传代的重组菌9株。

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The splicing system is a language generative mechanism based on splicing operations, where splicing operations are the mathematical abstractions of DNA strands recombinant behaviors under restriction exonucleases, endonucleases, DNA ligases and DNA polymerases.

剪接系统是将剪接运算当作基本算子的一种语言生成器，其中剪接运算是对在限制性内切酶、DNA连接酶、DNA聚合酶和外切酶作用下，DNA链进行重组过程的数学抽象。

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DNA vaccine is a new vaccine after live attenuated, whole killed, subunit and recombinant one, etc. It's better than them at efficiency, safety and ease to produce.

DNA疫苗是继减毒疫苗、灭活疫苗、亚单位疫苗和重组多肽疫苗之后的又一新型疫苗，比其它疫苗高效、安全且易于大量生产。

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DNA of engineering bacteria of recombinant double functions hirudin were labeled by Digoxin and used as probe for dot hybridization to detect residual DNA in semi-finished products of recombinant double functions hirudin for injection. At the same time, specificity and sensitivity experiments were performed.

为检测注射用重组双功能水蛭素半成品中的DNA残留量，应用地高辛标记重组双功能水蛭素工程菌DNA，并以此为DNA探针进行点杂交，同时做特异性及灵敏度实验。

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In conclusion, sufficient DNA for PCR can be quickly extracted from transgenic rice food by alkaline lysis method. The stability of recombinant DNA in transgenic was different under different processing conditions.

由此可知，碱处理法能够快速、有效的提取转基因稻米制品中DNA，转基因稻米中的各种外源成分在不同加工条件下的稳定性不同。

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There is a great deal of interest in the development of recombinant vector, DNA and protein subunit vaccines.

利用分子生物技术也研究出了很多基因工程疫苗比如基因工程活载体疫苗、亚单位疫苗和DNA疫苗等。

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Objective To explore the pilot fermentation procedure of recombinant E. coli strain containing two plasmids as therapeutic HBV DNA vaccine.

目的研究双质粒HBV dna疫苗工程菌的中试发酵工艺。

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